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Test Code VWFACM von Willebrand Factor Activity, Plasma

Additional Codes

EPIC EAP  Mayo
LAB2635 VWACT


Ordering Guidance


This activity assay is most effective when it is combined with measurement of von Willebrand factor antigen and factor VIII coagulant activity, preferably as a panel of tests with reflexive testing and interpretive reporting. See AVWPR / von Willebrand Disease Profile, Plasma.



Additional Testing Requirements


F8A / Coagulation Factor VIII Activity Assay, Plasma and VWAG / von Willebrand Factor Antigen, Plasma are recommended in conjunction with this test (von Willebrand activity).



Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial (polypropylene preferred)

Specimen Volume: 2 mL Platelet-poor plasma in 2 vials, each containing 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

3. Centrifuge, transfer all plasma into a polypropylene vial, and centrifuge plasma again.

4. Aliquot plasma (1-2 mL per aliquot) into 2 separate polypropylene vials leaving 0.25 mL in the bottom of centrifuged vial.

5. Immediately freeze plasma (no longer than 4 hours after collection) at -20° C or, ideally, ≤-40° C.

6. Send specimens in the same shipping container.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Diagnosis of von Willebrand disease (VWD) and differentiation of VWD subtypes or differentiation of VWD from hemophilia A

 

Monitoring therapeutic efficacy of treatment with DDAVP (desmopressin) or von Willebrand factor concentrates in patients with VWD

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RIST Ristocetin Cofactor, P No No

Method Name

Latex Immunoassay

Reporting Name

von Willebrand Factor Activity, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

Platelet-poor plasma: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Day(s) Performed

Monday through Saturday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85397

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VWACT von Willebrand Factor Activity, P 68324-3

 

Result ID Test Result Name Result LOINC Value
VWACT von Willebrand Factor Activity, P 68324-3

Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.