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Test Code TESTGM Testosterone, Total and Free, Serum

Additional Codes

EPIC EAP  Mayo
LAB173 TGRP

Reporting Name

Testosterone, Total and Free, S

Useful For

Alternative, second-level test for suspected increases or decreases in physiologically active testosterone:

-Assessment of androgen status in cases with suspected or known sex hormone-binding globulin-binding abnormalities

-Assessment of functional circulating testosterone in early pubertal boys and older men

-Assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism but normal total testosterone levels

-Monitoring testosterone therapy or antiandrogen therapy

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRTST Testosterone, Free, S No Yes
TTST Testosterone, Total, S Yes Yes

Method Name

FRTST: Equilibrium Dialysis/Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

TTST: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum Red


Ordering Guidance


This is a second-level test for suspected increases or decreases in physiologically active testosterone. The preferred test for assessment of active testosterone is TTBS / Testosterone, Total and Bioavailable, Serum.



Necessary Information


Patient's age and sex are required.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 2.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Frozen  60 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Special Instructions

Day(s) Performed

Monday through Saturday

CPT Code Information

84402

84403

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TGRP Testosterone, Total and Free, S 58952-3

 

Result ID Test Result Name Result LOINC Value
3631 Testosterone Free 2991-8
8533 Testosterone, Total, S 2986-8

Report Available

3 to 8 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.