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HelpTest Code NSAIP Neurosyphilis IgG Antibody Index with VDRL, Serum and Spinal Fluid
Ordering Guidance
This test should be ordered in patients with suspected neurosyphilis who are confirmed seropositive in blood.
For syphilis testing on serum, order SYPH1 / Syphilis IgG with Reflex, Enzyme Immunoassay, Serum or SYPH2 / Syphilis IgG with Reflex, Enzyme Immunoassay, Serum.
Additional Testing Requirements
Although a small percentage of patients with neurosyphilis may be seronegative, it is recommended that all patients tested by this assay are first confirmed seropositive for syphilis in blood.
Specimen Required
Both spinal fluid (CSF) and serum are required for this test. CSF and serum must be collected within a maximum of 24 hours of each other.
Specimen Type: Spinal fluid
Container/Tube: Sterile vial
Specimen Volume: 2.2 mL
Collection Instructions:
1. The spinal fluid (CSF) specimen must be collected within 24 hours of the serum specimen, preferably at the same time.
2. The CSF aliquot should be from the second, third, or fourth CSF vial collected during the lumbar puncture. Do not submit CSF from the first vial due to the possibility of blood contamination, which will cause specimen rejection.
3. Label vial as spinal fluid or CSF.
4. Band CSF specimen together with the serum sample.
Specimen Type: Serum
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2.2 mL
Collection Instructions:
1. Within 24 hours of collection of the spinal fluid specimen, a serum specimen must also be collected, preferably at the same time.
2. Centrifuge and aliquot serum into a plastic vial.
3. Label tube as serum.
4. Band serum specimen together with the CSF sample.
Useful For
Aid in the diagnosis of neuroinvasive syphilis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| NSCSF | Neurosyphilis IgG Screen, CSF | No | Yes |
| NSSER | Neurosyphilis IgG, S | No | No |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| NSAI | Neurosyphilis IgG, Ab Index | No | No |
| VDSFT | VDRL Titer,CSF | No | No |
Method Name
NSCSF, NSAI: Enzyme-Linked Immunosorbent Assay (ELISA)
NSSER: Technical Interpretation
VDSFT: Flocculation/Agglutination
Reporting Name
Neurosyphilis IgG Ab Indx w/VDRLSpecimen Type
CSFSerum
Specimen Minimum Volume
Spinal fluid: 1.5 mL; Serum: 1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| CSF | Refrigerated (preferred) | 10 days |
| Frozen | 10 days | |
| Serum | Refrigerated (preferred) | 10 days |
| Frozen | 10 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Spinal fluid (CSF) contaminated with blood | Reject |
Day(s) Performed
Monday through Friday, Sunday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86780
82784 x 2-(if appropriate)
82040-(if appropriate)
86780 x 2-(if appropriate)
82042-(if appropriate))
86593-(if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| NSAIP | Neurosyphilis IgG Ab Indx w/VDRL | 58031-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| NSSER | Neurosyphilis IgG, S | 69048-7 |
| NSY1 | Neurosyphilis IgG, CSF | 58031-6 |
| NSY2 | Neurosyphilis IgG Interp | 69048-7 |