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Test Code MPNRM Myeloproliferative Neoplasm, JAK2 V617F with Reflex to CALR and MPL, Varies

Additional Codes

EPIC EAP  Mayo
LAB6504 MPNR

Useful For

Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder

 

Evaluating for variants in JAK2, CALR, and MPL genes in an algorithmic process

Reporting Name

MPN (JAK2 V617F, CALR, MPL) Reflex

Specimen Type

Varies


Shipping Instructions


Specimen must arrive within 7 days of collection.



Necessary Information


The following information is required:

1. Pertinent clinical history

2. Clinical or morphologic suspicion

3. Date of collection

4. Specimen source



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Whole Blood

Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)

Specimen Volume: 3 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as whole blood.

Specimen Stability Information: Ambient (preferred)/Refrigerate

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) or yellow top (ACD solution B)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.

Specimen Stability Information: Ambient (preferred)/Refrigerate

 

Specimen Type: Extracted DNA from whole blood or bone marrow

Container/Tube: 1.5- to 2-mL tube

Specimen Volume: Entire specimen

Collection Instructions:

1. DNA must be extracted from blood or bone marrow within 7 days of collection.

2. Label specimen as extracted DNA and source of specimen

3. Provide volume and concentration of the DNA.

Specimen Stability Information: Frozen (preferred) 1 year/Refrigerate/Ambient

Additional Information: DNA must be extracted in a CLIA-certified laboratory or equivalent and must be extracted from a specimen type listed as acceptable for this test (including applicable anticoagulants). We cannot guarantee that all extraction methods are compatible with this test. If testing fails, one repeat will be attempted, and if unsuccessful, the test will be reported as failed and a charge will be applied.


Specimen Minimum Volume

Blood, bone marrow: 0.5 mL; Extracted DNA: 50 mcL at 20 ng/mcL concentration

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies 7 days

Reject Due To

Gross hemolysis Reject
Paraffin-embedded bone marrow aspirate clot or biopsy blocks Reject
Slides Reject
Paraffin shavings Reject
Moderately to severely clotted Reject

Day(s) Performed

Monday through Friday

Report Available

7 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant
81219-CALR (calreticulin) (eg, myeloproliferative disorders), gene analysis, common variants in exon 9 (if appropriate)
81339-MPL (MPL proto-oncogene, thrombopoietin receptor) (eg, myeloproliferative disorder) gene analysis; sequence analysis, exon 10 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MPNR MPN (JAK2 V617F, CALR, MPL) Reflex In Process

 

Result ID Test Result Name Result LOINC Value
39725 MPNR Result No LOINC Needed
36687 Final Diagnosis 22637-3

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
CALX CALR, Gene Mutation, Exon 9, Reflex No, (bill only) No
MPLR MPL Exon 10 Mutation Detection, R No, (bill only) No

Method Name

Quantitative Polymerase Chain Reaction (qPCR)

Forms

If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

UnityPoint Health-Saint Luke's Sioux City Note:

Last Updated: 02/09/2021  LM