Specimen Requirements

Container/Tube Type: 

Light Blue (Citrate)

Collection Information: 

Tube must be filled to a 1:9 ratio- see tube label for proper amount of blood

Transport Information: 

2.7 mL blood in a 3.0 mL tube or 2(two) 1.8 mL blood in a 2.0 mL tube

Minimum Sample Volume: 

0.25 mL of frozen plasma

Remarks: 

3.2% buffered sodium citrate is the only acceptable anticoagulant concentration for this testing. Tube should be inverted several times immediately after collection, mixing blood and anticoagulant, to prevent clotting. Tube must be filled with appropriate volume to obtain the proper 9:1 ratio blood to anticoagulant-see tube label. Specimen must be delivered immediately to the laboratory on ice or the plasma should be separated from the cells and immediately frozen.

Unacceptable Conditions: 

Clotted, Hemolyzed or Does not meet collection, storage or transport requirements

Stability Comments: 

Platelet Poor Plasma
Centrifuge Specimen
Recommended centrifuge speed is 1500 x G for at least 15 minutes. Be careful that the tubes are evenly balanced when placing them into the centrifuge. When the centrifuge stops, carefully remove and rack tubes. Be aware that an unbalanced centrifugation, speed too slow, or centrifugation for too short of a spin time may all lead to an increase number of cells in the plasma which could contribute to a clotted specimen or erroneous results.
Remove Plasma
To remove plasma, start from the top, gently draw specimen into pipette as you go further down tube. Leaving approximately 0.5mL of plasma will insure that you do not disturb the buffy coat and cell layer.
Second Spin
Plasma separated from cells goes back into the centrifuge for a second spin. Balance and centrifuge the tubes. Failure to perform a second spin will result in plasma containing too many platelets which interfere with some coagulation testing and is a contributing factor for a clotted specimen.
Transfer Plasma to Aliquot Tubes
Pipette plasma from second spin tube into labeled aliquot tubes without disturbing the bottom 0.5mL.
Specimen Storage
Promptly freeze specimens after processing.