Useful For

Simultaneous high-sensitivity determination of serum estrone and estradiol levels

Situations requiring either higher sensitivity estradiol measurement, estrone measurement, or both, including

-As part of the diagnosis and workup of precocious and delayed puberty in females and, to a lesser degree, males

-As part of the diagnosis and workup of suspected disorders of sex steroid metabolism, (eg, aromatase deficiency and 17 alpha-hydroxylase deficiency)

-As an adjunct to clinical assessment, imaging studies, and bone mineral density measurement in the fracture risk assessment of postmenopausal women and, to a lesser degree, older men

-Monitoring low-dose female hormone replacement therapy in postmenopausal women

-Monitoring antiestrogen therapy (eg, aromatase inhibitor therapy)

Applications that require moderately sensitive measurement of estradiol including:

-Evaluation of hypogonadism and oligo-amenorrhea in females

-Assessing ovarian status, including follicle development, for assisted reproduction protocols (eg, in vitro fertilization)

In conjunction with luteinizing hormone measurements, monitoring of estrogen replacement therapy in hypogonadal premenopausal women

Evaluation of feminization, including gynecomastia, in males

Diagnosis of estrogen-producing neoplasms in males, and, to a lesser degree, females