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Test Code DMAID Mycobacterium abscessus Complex Subspecies Identification, Next-Generation Sequencing


Ordering Guidance


This test is used to identify the subspecies of Mycobacterium abscessus from a known M abscessus isolate.

 

For the identification of M abscessus (not the subspecies) from culture isolate growth, order CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies.



Shipping Instructions


1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and suspected organism identification are required.



Specimen Required


Supplies: Infectious Container, Large (T146)

Specimen Type: Mycobacterium abscessus isolate growing in pure culture

Container/Tube:

Preferred: Solid slant medium (eg. Middlebrook 7H10, 7H11 or Lowenstein Jensen agar)

Acceptable: Broth medium (eg. Mycobacteria Growth Indicator Tube, 7H9 broth, BACT/ALERT MP or VersaTREK bottle). Note: Broth specimens will require subculture which will delay results.

Specimen Volume:

Solid slant medium: Isolate with visible growth

Broth medium: Greater than or equal to 3 mL of broth culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.


Useful For

Determining the subspecies of a Mycobacterium abscessus complex culture isolate

Method Name

Next-Generation Sequencing (NGS)

Reporting Name

M abscessus subspecies ID, NGS

Specimen Type

Varies

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject
Mixed culture Reject

Day(s) Performed

Monday through Friday

Report Available

7 to 14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87153

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DMAID M abscessus subspecies ID, NGS 112278-7

 

Result ID Test Result Name Result LOINC Value
DMAID M abscessus subspecies ID, NGS 112278-7