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Test Code CHF8 Chromogenic Factor VIII Activity Assay, Plasma

Additional Codes

Hospital Order Code
Lab2608   MISMGO


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, a coagulation consultation is recommended.



Necessary Information


 



Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL Platelet-poor plasma

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

5. Immediately freeze plasma  (no longer than 4 hours after collection) at -20° C or, ideally, at -40° C or below.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Monitoring coagulation factor replacement therapy of selected extended half-life coagulation factor replacements

 

Aiding in the diagnosis of hemophilia A using a 2-stage assay, especially when the 1-stage assay result was normal

Method Name

Chromogenic

Reporting Name

Chromogenic FVIII, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

Platelet-poor plasma: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK
IV heparin contamination Reject

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

85130

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHF8 Chromogenic FVIII, P 49865-9

 

Result ID Test Result Name Result LOINC Value
CHF8 Chromogenic FVIII, P 49865-9

Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.