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Test Code CCSF Culture, CSF, with Gram Stain

Additional Codes

Epic EAP
LAB268 CSF culture with gram stain 

Specimen Requirements

Container/Tube Type: 

Sterile CSF Container

 

Collection Information: 

Cerebrospinal Spinal Fluid

 

Transport Information: 

3.0 mL fluid. Aseptic technique must be used in obtaining, and transport of specimen. If unusual, fastidious, or slow-growing organisms are suspected, inform Microbiology. Be sure to comment if specific organisms are suspected. A gram stain is routinely performed on this specimen type. Transport to the laboratory immediately.

 

Remarks: 

Identification and susceptibility tests are billed separately from culture.

 

Unacceptable Conditions: 

Specimen in a nonsterile container, more than 2 hours old or Does not meet collection, storage or transport requirements. For hard to collect specimens, consult the Microbiology Manager before rejecting.

 

Stability Comments: 

Submit samples at ambient temperature only.

Stability

Refrigerated Stability: 

NOT Acceptable

 

Frozen Stability: 

NOT Acceptable

 

Ambient Stability: 

24 hours

Performing Laboratory

Pathology Laboratory, UnityPoint Health, Des Moines

CPT

87070 - culture

87205 - gram stain

Methodology

Organism Identification is performed by biochemical test and/or Bruker MALDI-TOF mass spectrometry RUO Database. Organism sensitivities (AST) are performed as applicable using Broth Microdilution and/or Gradient diffusion strips using FDA approved methodology and CLSI guidelines.  Performance has been evaluated and verified by UnityPoint Health - Des Moines, Pathology Laboratory.

Performance

Setup daily, 24 hours a day

 

Analytical Time: 

4 days
Analytical time listed is the average time for a negative culture. Average analytical time may vary for positive, mixed, and complex cultures. Contact the lab for specific questions.

 

Testing Laboratory: 

Pathology Laboratory

 

Laboratory Section: 

Microbiology

Alias

Aerobic Bacteria Culture, CSF, Aerobic Culture