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Test Code BOHB Beta-Hydroxybutyrate

Additional Codes

Epic EAP
LAB2551 Beta-Hydroxybutyrate 

Specimen Requirements

Preferred Collection Container: 

Light Green (Lithium Heparin Plasma Separator Tube)
SST (Serum Separator)

 

Also Acceptable Collection Container: 

Red (No Additive)
Green No Gel (Lithium Heparin)

 

Collection Information: 

Collect blood in a light green lithium heparin plasma separator or serum separator tube and centrifuge within 2 hours of collection.

 

Transport Information: 

Centrifuge and aliquot 2.0 mL of serum or plasma into a plastic transport vial. Send refrigerated. 

 

Minimum Sample Volume: 

0.5 mL of serum or plasma (approximately 1.0 mL of whole blood based upon a normal patient hematocrit)

Performing Laboratory

Unity Point-Sioux City-Chemistry

CPT

82010

Methodology

Photometric, B-Hydroxybutyrate Dehydrogenase (B-HBDH) on COBAS 6000

Specimen Stability

Refrigerated Stability: 

30 days - Preferred

 

Frozen Stability: 

30 months  (Only freeze once)

 

Ambient Stability: 

30 hours

Performance

Daily

 

Useful For

Monitoring therapy for diabetic ketoacidosis

 

Investigating the differential diagnosis of any patient presenting to the emergency room with hypoglycemia, acidosis, suspected alcohol ingestion, or an unexplained increase in the anion gap

 

In pediatric patients, the presence or absence of ketonemia/uria is an essential component in the differential diagnosis of inborn errors of metabolism

 

Serum beta-hydroxybutyrate is a key parameter monitored during controlled 24-hour fasts

Specimen Linearity

Specimens are linear to 47.8

Instrument will automatically dilute samples x 2.  If still out side linearity report as greater than 95.6 mg/dL. 

If physician requires an endpoint, please dilute X 10 manually with water and report.