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Test Code ANAWR Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Additional Codes

Hospital Ordering Code
LAB5463  Ana By IFA W Reflex To Immunoassay And Path Interp

Specimen Requirements

Preferred Collection Container: 

SST (Serum Separator)

 

Also Acceptable Collection Container: 

Red (No Additive)

 

Collection Information: 

Collect blood in a serum separator tube. (Serum red top also acceptable).

 

Transport Information: 

Allow blood to clot at room temperature. Centrifuge and aliquot 1.5 mL of serum to a plastic transport vial. Send refrigerated.

 

Minimum Sample Volume: 

1.0 mL of serum (approximately 2.0 mL of whole blood based upon a normal patient hematocrit)

 

Remarks: 

ANA samples are screened at a titer of 1:80. All samples that are positive are titered and the titer and pattern will be reported. All positives include a Pathologist's interpretation.

 

Unacceptable Conditions: 

Plasma, gross hemolysis, icterus, lipemia, bacterial growth.

Stability

Refrigerated Stability: 

7 days

 

Frozen Stability: 

Indefinitely

Performing Laboratory

Pathology Laboratory, UnityPoint Health, Des Moines

CPT

86038 if negative;

If positive 86038 x2, 86039, 86235 x10, 83520

Methodology

Immunofluorescence and Multplex Flow Immunoassay

Alias

ANA (antinuclear antibodies), ANA, ANA1, ANA2, Antinuclear antibodies (ANA), HEp-2 substrate, serum, Antinuclear Antibody, Serum, FANA (fluorescent antinuclear antibodies), Hep-2, LE (lupus erythematosus) prep; ANA WR

Performance

Monday through Friday

 

Analytical Time: 

1 to 3 days

 

Testing Laboratory: 

Pathology Laboratory

 

Laboratory Section: 

Core Laboratory