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Test Code ANAWR Antinuclear Antibodies (ANA) by Immunofluorescence with Pathologist Interpretation and Reflex to ANA10 Battery

Additional Codes

Hospital Ordering Code
LAB5463  Ana By IFA W Reflex To Immunoassay And Path Interp

Specimen Requirements

Preferred Collection Container: 

SST (Serum Separator)

 

Also Acceptable Collection Container: 

Red (No Additive)

 

Collection Information: 

Collect blood in a serum separator tube. (Serum red top also acceptable).

 

Transport Information: 

Allow blood to clot at room temperature. Centrifuge and aliquot 1.5 mL of serum to a plastic transport vial. Send refrigerated.

 

Minimum Sample Volume: 

1.0 mL of serum (approximately 2.0 mL of whole blood based upon a normal patient hematocrit)

 

Remarks: 

ANA samples are screened at a titer of 1:80. All samples that are positive are titered and the titer and pattern will be reported. All positives include a Pathologist's interpretation.

 

Unacceptable Conditions: 

Plasma, gross hemolysis, icterus, lipemia, bacterial growth.

Stability

Refrigerated Stability: 

7 days

 

Frozen Stability: 

Indefinitely

CPT

86038 if negative;

If positive 86038 x2, 86039, 86235 x10, 83520

Performing Laboratory

Pathology Laboratory, UnityPoint Health, Des Moines

Methodology

Immunofluorescence and Multplex Flow Immunoassay

Alias

ANA (antinuclear antibodies), ANA, ANA1, ANA2, Antinuclear antibodies (ANA), HEp-2 substrate, serum, Antinuclear Antibody, Serum, FANA (fluorescent antinuclear antibodies), Hep-2, LE (lupus erythematosus) prep; ANA WR

Performance

Monday through Friday

 

Analytical Time: 

1 to 3 days

 

Testing Laboratory: 

Pathology Laboratory

 

Laboratory Section: 

Core Laboratory